Tumor Vascular Heterogeneity and The Impact of Subtumoral Nanoemulsion Biodistribution
Jaqueline Vaz de Oliveira, Márcia Cristina Oliveira da Rocha, Ailton A. Sousa-Junior, Mosar Corrêa Rodrigues, Gabriel Ribeiro Farias, Patrícia Bento da Silva, Sônia Nair Báo, Andris F. Bakuzis, Ricardo Bentes Azevedo, P.C. Morais, Luís Alexandre Muehlmann, João Paulo Figueiró Longo
Abstract
Aim: Investigate the heterogeneous tumor tissue organization and examine how this condition can interfere with the passive delivery of a lipid nanoemulsion in two breast cancer preclinical models (4T1 and Ehrlich). Materials & methods: The authors used in vivo image techniques to follow the nanoemulsion biodistribution and microtomography, as well as traditional histopathology and electron microscopy to evaluate the tumor structural characteristics. Results & conclusion: Lipid nanoemulsion was delivered to the tumor, vascular organization depends upon the subtumoral localization and this heterogeneous organization promotes a nanoemulsion biodistribution to the highly vascular peripherical region. Also, the results are presented with a comprehensive mathematical model, describing the differential biodistribution in two different breast cancer models, the 4T1 and Ehrlich models.