Clinical Evaluation of Metagenomic Next-Generation Sequencing Method for the Diagnosis of Suspected Ascitic Infection in Patients with Liver Cirrhosis in a Clinical Laboratory
Hao-Xin Wu, Feili Wei, Wei Zhang, Jie Han, Shan Guo, Zheng Wang, Dexi Chen, Wei Hou, Zhong Hu
Abstract
This study established and evaluated a complete metagenomics next-generation sequencing assay to improve the diagnosis of suspected ascitic infection in a clinical laboratory affiliated with a hospital. The assay is superior to traditional culture testing and will aid in the early and accurate identification of pathogens, particularly in patients with polymicrobial infections. This assay is also essential for precision therapy and can reduce the incidence of drug resistance stemming from irrational use of antibiotics.
Topics & Concepts
CirrhosisMetagenomicsAscitesMedicineInternal medicinePathogenAntibioticsClinical PracticeProspective cohort studyGastroenterologyBiologyMicrobiologyImmunologyFamily medicineGeneBiochemistryAmoebic Infections and TreatmentsLiver Disease and TransplantationClostridium difficile and Clostridium perfringens research