3D tracking of extracellular vesicles by holographic fluorescence imaging
Matz Liebel, Jaime Ortega Arroyo, Vanesa Sanz Beltrán, Johann Osmond, Ala Jo, Hakho Lee, Romain Quidant, N.F. van Hulst
Abstract
-range of 8 mm. As a proof-of-concept biological application, we image the 3D motion of extracellular vesicles (EVs) inside live cells. At short time scales (<4 s), we resolve near-isotropic 3D diffusion and directional transport. For longer lag times, we observe a transition toward anisotropic motion with the EVs being transported over long distances in the axial plane while being confined in the horizontal dimension.
Topics & Concepts
Tracking (education)HolographyExtracellular vesiclesOpticsFluorescenceIsotropyVesicleMicroscopyFluorescence-lifetime imaging microscopyFluorescence microscopeDigital holographyPlane (geometry)Resolution (logic)PhysicsBiophysicsChemistryComputer scienceBiologyArtificial intelligenceGeometryMathematicsPedagogyBiochemistryMembraneCell biologyPsychologyDigital Holography and MicroscopyAdvanced Fluorescence Microscopy TechniquesMicrofluidic and Bio-sensing Technologies