Immunochromatographic assays for ultrasensitive and high specific determination of enrofloxacin in milk, eggs, honey, and chicken meat
Xianlu Lei, Xinxin Xu, Liqiang Liu, Hua Kuang, Liguang Xu, Chuanlai Xu
Abstract
) were 0.16-0.82 ng/g, 0.24-1.8 ng/g, 0.25-3.6 ng/g, and 0.61-3.9 ng/g by colloidal gold-immunochromatographic sensor (CG-ICS) analysis, and 0.022-0.42 ng/g, 0.054-0.42 ng/g, 0.069-1.4 ng/g, and 0.19-2.2 ng/g by Eu-fluorescence-immunochromatographic sensor (EF-ICS) analysis. The intraassay and interassay recovery rates were 88.9 to 108.5% with coefficients of variation of 1.3 to 7.0% by CG-ICS analysis, and 88.6 to 113.6% with coefficients of variation of 1.3 to 8.1% by EF-ICS analysis. Thus, our newly developed ICS are sensitive and reliable, providing an option for rapid quantitative detection of enrofloxacin in food samples.
Topics & Concepts
EnrofloxacinFood sciencePoultry meatChemistryBiologyMicrobiologyAntibioticsCiprofloxacinAntibiotics Pharmacokinetics and EfficacyBiosensors and Analytical DetectionAntibiotic Resistance in Bacteria