Mining Marine Metagenomes Revealed a Quorum-Quenching Lactonase with Improved Biochemical Properties That Inhibits the Food Spoilage Bacterium Pseudomonas fluorescens
Rami Haramati, Shlomit Dor, David Gurevich, Doron Levy, Dekel Freund, Giora Rytwo, Itai Sharon, Livnat Afriat‐Jurnou
Abstract
Our results emphasize the potential of sequence and structure-based identification of new QQ enzymes from environmental metagenomes, such as from the ocean, with improved stability or activity. The findings also suggest that purified QQ enzymes can present new strategies against food spoilage, in addition to their recognized involvement in inhibiting bacterial pathogen virulence factors. Future studies on the delivery and safety of enzymatic QQ strategy against bacterial food spoilage should be performed.
Topics & Concepts
Pseudomonas fluorescensQuorum sensingVirulenceFood spoilageQuorum QuenchingBiologyMicrobiologyEnzymeBacteriaPathogenPseudomonasMarine bacteriophagePseudomonadalesGeneBiochemistryGeneticsBacterial biofilms and quorum sensingEnzyme Production and CharacterizationMicrobial Community Ecology and Physiology