Pulsed laser assisted high-throughput intracellular delivery in hanging drop based three dimensional cancer spheroids
Pallavi Gupta, Srabani Kar, Ashish Kumar, Fan‐Gang Tseng, Shantanu Pradhan, Pallab Sinha Mahapatra, Tuhin Subhra Santra
Abstract
particles per ml) resulted in the successful delivery of PI dye into cancer cells. This technique ensured high delivery efficiency (89.6 ± 2.8%) while maintaining high cellular viability (97.4 ± 0.4%), thereby validating the applicability of this technique for intracellular delivery. The optoporation-based strategy can enable high-throughput single cell manipulation, is scalable towards larger 3D tissue constructs, and may provide translational benefits for the delivery of anti-cancer therapeutics to tumors.
Topics & Concepts
SpheroidDrop (telecommunication)IntracellularThroughputLaserMaterials scienceNanotechnologyBiophysicsChemistryComputer scienceCell biologyOpticsPhysicsBiologyTelecommunicationsBiochemistryIn vitroWireless3D Printing in Biomedical ResearchMicrofluidic and Bio-sensing TechnologiesElectrohydrodynamics and Fluid Dynamics