A bi-kinase module sensitizes and potentiates plant immune signaling
Philipp Köster, Gefeng He, Changyun Liu, Qiuyan Dong, Katarina Hake, Ina Schmitz-Thom, Paulina Heinkow, Jürgen Eirich, Lukas Wallrad, Kenji Hashimoto, Stefanie Schültke, Iris Finkemeier, Tina Romeis, Jörg Kudla
Abstract
Systemic signaling is an essential hallmark of multicellular life. Pathogen encounter occurs locally but triggers organ-scale and organismic immune responses. In plants, elicitor perception provokes systemically expanding Ca 2+ and H 2 O 2 signals conferring immunity. Here, we identify a Ca 2+ sensing bi-kinase module as becoming super-activated through mutual phosphorylation and as imposing synergistically enhanced NADPH oxidase activation. A combined two-layer bi-kinase/substrate phospho-code allows for sensitized signaling initiation already by near-resting elevations of Ca 2+ concentration. Subsequently, it facilitates further signal wave proliferation with minimal Ca 2+ amplitude requirement, triggering protective defense responses throughout the plant. Our study reveals how plants build and perpetuate trans-cellular immune signal proliferation while avoiding disturbance of ongoing cellular signaling along the path of response dissemination.