Temperature- and pH-responsive poly(<i>N</i>-isopropylacrylamide-<i>co</i>-methacrylic acid) microgels as a carrier for controlled protein adsorption and release
Priyanshi Agnihotri, Sangeeta, Shikha Aery, Abhijit Dan
Abstract
ions, salting out of BSA and aggregate formation of the microgels. The adsorbed BSA could be controllably released by adjusting the temperature and pH of the experiment, and with the help of sodium dodecyl sulphate (SDS) addition so as to eliminate each interaction between BSA and the microgels. Thus, this study can be useful to design a stimuli-responsive microgel-based carrier for controlled release of proteins.
Topics & Concepts
AdsorptionMethacrylic acidChemistryPoly(N-isopropylacrylamide)Bovine serum albuminIonic strengthPoly(methacrylic acid)Chemical engineeringIonic bondingHydrogen bondHydrophobic effectSwellingPolyelectrolyteDiffusionProtein adsorptionPolymer chemistryPolymerIonChromatographyMoleculeOrganic chemistryAqueous solutionCopolymerThermodynamicsPhysicsEngineeringHydrogels: synthesis, properties, applicationsPolymer Surface Interaction Studies3D Printing in Biomedical Research