High-Resolution Ion-Mobility-Enabled Peptide Mapping for High-Throughput Critical Quality Attribute Monitoring
James R. Arndt, Kelly L. Wormwood Moser, Gregory Van Aken, Rory M. Doyle, Tatjana Talamantes, Daniel DeBord, Laura Maxon, George Stafford, John C. Fjeldsted, Bryan Miller, Melissa Sherman
Abstract
-extracted HRIM arrival time distributions, showing good agreement with the reference liquid-phase separation. This study shows, for the first time, the analytical capability of HRIM using SLIM technology for enhancing peptide mapping workflows relevant to biopharmaceutical characterization.
Topics & Concepts
ChemistryChromatographyIsobaric processPeptideIon-mobility spectrometryMass spectrometryAnalytical Chemistry (journal)BiochemistryThermodynamicsPhysicsMass Spectrometry Techniques and ApplicationsProtein purification and stabilityAnalytical Chemistry and Chromatography