One-step genome editing of porcine zygotes through the electroporation of a CRISPR/Cas9 system with two guide RNAs
Maki Hirata, Manita Wittayarat, Fuminori Tanihara, Yoko Sato, Zhao Namula, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Takeshige Otoi
Topics & Concepts
CRISPRGuide RNABiologyGenome editingElectroporationCas9GeneticsZygoteEmbryoComputational biologyGeneEmbryogenesisCRISPR and Genetic EngineeringRNA regulation and diseaseViral Infectious Diseases and Gene Expression in Insects