Capillary Force-Driven Quantitative Plasma Separation Method for Application of Whole Blood Detection Microfluidic Chip
Xiaohua Fang, Cuimin Sun, Peng Dai, Zhaokun Xian, Wenyun Su, Chaowen Zheng, Dong Xing, Xiaotian Xu, Hui You
Abstract
Separating plasma or serum from blood is essential for precise testing. However, extracting precise plasma quantities outside the laboratory poses challenges. A recent study has introduced a capillary force-driven membrane filtration technique to accurately separate small plasma volumes. This method efficiently isolates 100-200 μL of pure human whole blood with a 48% hematocrit, resulting in 5-30 μL of plasma with less than a 10% margin of error. The entire process is completed within 20 min, offering a simple and cost-effective approach to blood separation. This study has successfully addressed the bottleneck in self-service POCT, ensuring testing accuracy. This innovative method shows promise for clinical diagnostics and point-of-care testing.