Probing protein aggregation at buried interfaces: distinguishing between adsorbed protein monomers, dimers, and a monomer–dimer mixture <i>in situ</i>
Tieyi Lu, Wen Guo, Prathamesh M. Datar, Yue Xin, E. Neil G. Marsh, Zhan Chen
Abstract
SFG spectra analyzed by the developed Hamiltonian method show that adsorbed BSA molecules on silicone oil are dimers. On treatment with dithiothreitol, some BSA dimers dissociate, resulting in 60% dimer and 40% monomer on the silicone oil surface.
Topics & Concepts
MonomerDimerIn situChemistryAdsorptionCrystallographyPolymerOrganic chemistrySpectroscopy and Quantum Chemical StudiesPolymer Surface Interaction StudiesLipid Membrane Structure and Behavior