Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry
Zhaoyuan Liu, Yaqi Gu, Amanda Shin, Shuangyan Zhang, Florent Ginhoux
Abstract
Myeloid cells, including dendritic cells (DCs), granulocytes, monocytes, monocyte-derived cells and macrophages, are important players in the immune response, but their identification is not as clear as lymphocytes, especially in tissues. This protocol details the step-by-step procedure for the analysis of myeloid populations in various mouse tissues by flow cytometry. For complete details on the use and execution of this protocol, please refer to Liu et al. (2019).
Topics & Concepts
Flow cytometryMyeloid cellsMyeloidMonocyteImmune systemCell biologyImmunologyBiologyImmune cells in cancerNeuroinflammation and Neurodegeneration MechanismsImmune Cell Function and Interaction