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Machine learning in point-of-care testing: innovations, challenges, and opportunities

Gyeo‐Re Han, Artem Goncharov, Merve Eryılmaz, Shun Ye, Barath Palanisamy, Rajesh Ghosh, Fabio Lisi, Elliott Rogers, David Guzman, Defne Yigci, Savaş Taşoğlu, Dino Di Carlo, Keisuke Goda, Rachel A. McKendry, Aydogan Özcan

2025Nature Communications184 citationsDOIOpen Access PDF

Abstract

The landscape of diagnostic testing is undergoing a significant transformation, driven by the integration of artificial intelligence (AI) and machine learning (ML) into decentralized, rapid, and accessible sensor platforms for point-of-care testing (POCT). The COVID-19 pandemic has accelerated the shift from centralized laboratory testing but also catalyzed the development of next-generation POCT platforms that leverage ML to enhance the accuracy, sensitivity, and overall efficiency of point-of-care sensors. This Perspective explores how ML is being embedded into various POCT modalities, including lateral flow assays, vertical flow assays, nucleic acid amplification tests, and imaging-based sensors, illustrating their impact through different applications. We also discuss several challenges, such as regulatory hurdles, reliability, and privacy concerns, that must be overcome for the widespread adoption of ML-enhanced POCT in clinical settings and provide a comprehensive overview of the current state of ML-driven POCT technologies, highlighting their potential impact in the future of healthcare. Recent years have seen an increasing shift from centralized laboratory diagnostics to decentralized point-of-care testing, a shift which has the potential to increase health equity. Here the authors provide their perspective on how the integration of machine learning and artificial intelligence with point-of-care technologies can - and could - support this transition

Topics & Concepts

Point-of-care testingComputer scienceLeverage (statistics)Coronavirus disease 2019 (COVID-19)ModalitiesHealth careSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Point of careRisk analysis (engineering)Systems engineeringArtificial intelligenceEngineeringMedicinePathologyDiseaseInfectious disease (medical specialty)Economic growthSociologyEconomicsSocial scienceBiosensors and Analytical DetectionSARS-CoV-2 detection and testingAdvanced Biosensing Techniques and Applications
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