Evaluation of Various <i>Escherichia coli</i> Strains for Enhanced Lycopene Production
Jun Ren, Junhao Shen, Thi Duc Thai, Min-gyun Kim, Seung Ho Lee, Wonseop Lim, Dokyun Na
Abstract
). The 16 lycopene strain titers diverged from 0 to 0.141 g/l, with MG1655 demonstrating the highest titer (0.141 g/l), while the SURE and W strains expressed the lowest (0 g/l) in an LB medium. When a 2 × YTg medium replaced the MG1655 culture medium, the titer further escalated to 1.595 g/l. These results substantiate that strain selection is vital in metabolic engineering, and further, that MG1655 is a potent host for producing lycopene and other carotenoids with the same lycopene biosynthetic pathway.
Topics & Concepts
LycopeneEscherichia coliCarotenoidMetabolic engineeringBiologyTiterStrain (injury)AntioxidantPhylotypeFood scienceMicrobiologyBiochemistryGeneGenetics16S ribosomal RNAAntibodyAnatomyMicrobial Metabolic Engineering and BioproductionPhotosynthetic Processes and MechanismsPlant biochemistry and biosynthesis