The influence of dysfunctional actin on polystyrene-nanotube-mediated mRNA nanoinjection into mammalian cells
Hao Zhe Yoh, Yaping Chen, Ali‐Reza Shokouhi, Helmut Thissen, Nicolas H. Voelcker, Roey Elnathan
Abstract
inhibiting actin polymerization and depolymerization, respectively (before and after the establishment of cell-PSNT interface). By inhibiting actin dynamics 12 h before cell-PSNT interfacing (pre-interface treatment), the mRNA delivery efficiencies were significantly reduced to ∼3% for Cyto D-treated samples and ∼1% for Jas-treated sample, as compared to their post-interface (2 h after cell-PSNT interfacing) counterpart (∼46% and ∼68%, respectively). The added flexibility of PSNTs have shown to help withstand mechanical breakage stemming from cytoskeletal forces in contrast to the SiNTs. Such findings will step-change our capacity to use programmable polymeric NTs in fundamental cellular processes related to intracellular delivery.