Enhancing cadmium stress resilience in chickpea ( <i>Cicer arietinum</i> L.) via exogenous melatonin application
Kiran Shehzadi, Muhammad Faisal Maqsood, Rehana Kanwal, Muhammad Shahbaz, Maria Naqve, Usman Zulfiqar, Muhammad Jamil, Noreen Khalid, Muhammad Fraz Ali, Walid Soufan
Abstract
Chickpea (Cicer arietinum L.) productivity is hindered by biotic and abiotic stresses, particularly heavy metal toxicity. The pot experiment was carried out at the botanical garden of The Islamia University of Bahawalpur, Bahawalpur-Pakistan. The experimental treatments comprised of following details: T0 = Control + 0 µM MT, T1 = Control + 15 µM MT, T2= Control + 30 µM MT, T3 = 100 µM Cd + 0 µM MT, T4 = 100 µM Cd + 15 µM MT and T5 = 100 µM Cd + 30 µM MT. A completely randomized design (CRD) with three replicates was used. Cd stress significantly reduced shoot fresh (51.3%) and dry weight (50.4%), total chlorophyll (53.6%), and shoot Ca2+ (56.6%). However, it increased proline (38.3%), total phenolics (74.2%), glycine betaine (46.4%), TSS (67.7%), TSP (50%), SOD (49.5%), POD (107%), and CAT (74.2%). Conversely, 30 µM MT improved shoot fresh (78.5%) and dry weight (76%), total chlorophyll (47%), SOD (26.5%), POD (35.8%), CAT (27.8%), proline (19%), TSS (24.5%), TSP (25.8%), and shoot Ca2+ (56.6%). Results indicated that MT enhanced photosynthetic pigments and antioxidant activities, maintained ion homeostasis, and reduces reactive oxygen species. Desi variety performed better than Kabuli, and 30 µM MT application effectively mitigated Cd toxicity.