Vascularized dental pulp regeneration using cell-laden microfiber aggregates
Qingqing Liang, Liang Cheng, Xiaojing Liu, Xiaotao Xing, Shixing Ma, Haisen Huang, Chao Liang, Lei Liu, Li Liao, Weidong Tian
Abstract
. Compared to a conventional mixture of DPSCs and HUVECs in GelMA hydrogel blocks, the aggregates of cell-laden microfibers generated more pulp-like tissue, blood vessels, and odontoblast-like cells that positively express DMP-1 and DSPP. To our knowledge, this is the first attempt to apply cell-laden MAs for pulp regeneration. Our study proposes a new solution to the challenge of pulp regeneration, which might promote the clinical translation and application of stem cell-based therapy.
Topics & Concepts
MicrofiberPulp (tooth)Regeneration (biology)Materials scienceSchematicDentistryComposite materialEngineeringCell biologyBiologyMedicineElectronic engineering3D Printing in Biomedical ResearchMesenchymal stem cell researchElectrospun Nanofibers in Biomedical Applications