Mobility capillary electrophoresis–native mass spectrometry reveals the dynamic conformational equilibrium of calmodulin and its complexes
Yi Zhao, Wenjing Zhang, Jie Hong, Lei Yang, Yuanyuan Wang, Feng Qu, Wei Xu
Abstract
ions did not affect the structural dynamics of CaM, while the binding of a peptide ligand would stabilize CaM, leading to the observation of a single, compact conformation of the resulting protein complex. A target recognition mechanism was also proposed based on these new findings, suggesting that CaM's interaction with targets may favor a conformational selection model. This enriches our understanding of the binding principles between CaM and its numerous targets.
Topics & Concepts
CalmodulinCapillary electrophoresisMass spectrometryChemistryHigh resolutionResolution (logic)ElectrophoresisBiophysicsChromatographyBiochemistryBiologyComputer scienceArtificial intelligenceRemote sensingGeologyEnzymeMass Spectrometry Techniques and ApplicationsProtein Structure and DynamicsMetabolomics and Mass Spectrometry Studies