2,5-Furandicarboxaldehyde as a bio-based crosslinking agent replacing glutaraldehyde for covalent enzyme immobilization
Chiara Danielli, Luuk M. van Langen, Deborah Boes, Fioretta Asaro, Serena Anselmi, Francesca Provenza, Monia Renzi, Lucia Gardossi
Abstract
The bio-based 2,5-furandicarboxaldehyde (diformylfuran, DFF) proved as effective as glutaraldehyde in the covalent immobilization of enzymes on amino-functionalized carriers. The binding is attributed to stable imine bond formation.
Topics & Concepts
GlutaraldehydeCovalent bondChemistryImineBifunctionalAqueous solutionPolymer chemistryOrganic chemistryCombinatorial chemistryCatalysisEnzyme Catalysis and ImmobilizationEnzyme Production and Characterizationbiodegradable polymer synthesis and properties