Dynamic Analysis of m6A Methylation Spectroscopy During Progression and Reversal of Hepatic Fibrosis
Zhongqi Cui, Nan Huang, Li Liu, Xue Li, Guohui Li, Yan Chen, Qi Wu, Jie Zhang, Shuping Long, Minyi Wang, Fenyong Sun, Yi Shi, Qiuhui Pan
Abstract
Aim: To dynamically analyze the differential m6A methylation during the progression and reversal of hepatic fibrosis. Materials & methods: We induced hepatic fibrosis in C57/BL6 mice by intraperitoneal injection of CCl4. The reversal model of hepatic fibrosis was established by stopping drug after continuous injection of CCl4. Dynamic m6A methylation was evaluated using MeRIP-Seq in the progression and reversal of hepatic fibrosis at different stages. Result: During the hepatic fibrosis, differential m6A methylation was mainly enriched in processes associated with oxidative stress and cytochrome metabolism, while differential m6A methylation was mainly enriched in processes associated with immune response and apoptosis in the hepatic fibrosis reversal. Conclusion: m6A methylation plays an important role in the progression and reversal of hepatic fibrosis.