Transient siRNA-mediated protein knockdown in mouse followed by feeding/starving cycle and liver tissue analysis
Lidia Wróbel, Farah H. Siddiqi, David C. Rubinsztein
Abstract
We present a protocol for in vivo siRNA-mediated knockdown of a gene of interest in mouse liver using systemic delivery via intravenous injection. We describe a step-by-step protocol for delivery of siRNA particles, with tips on how to optimize dosage. We detail steps for feeding/starving cycles as well as for liver tissue isolation, followed by gene expression analysis, measured at the mRNA and protein levels. For complete information on the generation and use of this protocol, please refer to Wrobel et al. (2020).
Topics & Concepts
Gene knockdownMessenger RNALiver tissueIn vivoMolecular biologyCell biologySmall interfering RNATransient (computer programming)GeneChemistryBiologyRNABiochemistryComputer scienceGeneticsEndocrinologyOperating systemRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniquesMolecular Biology Techniques and Applications