Effect of Vitamin D3 on Mitochondrial Biogenesis in Granulosa Cells Derived from Polycystic Ovary Syndrome.
Zahra Safaei, Shabnam Bakhshalizadeh, Mohammad Hossein Nasr‐Esfahani, Azadeh Akbari Sene, Vahid Najafzadeh, Mansoureh Soleimani, Reza Shirazi
Abstract
BACKGROUND: copy number, mitochondrial biogenesis, and membrane integrity of granulosa cells in a PCOS-induced mouse model. MATERIALS AND METHODS: copy number was also investigated by qPCR. The mitochondrial structure was evaluated by transmission electron microscopy (TEM). Hormonal levels were measured by an enzymelinked immunosorbent assay (ELISA) kit. RESULTS: copy number increased significantly in comparison to PCOS granulosa cells with no treatments. CONCLUSION: According to this study, we can conclude, vitamin D3 improves mitochondrial biogenesis and membrane integrity, mtDNA copy number in granulosa cells of PCOS mice which might improve follicular development and subsequently oocyte quality.