Intradermal delivery of receptor‐binding domain of <scp>SARS‐CoV‐2</scp> spike protein with dissolvable microneedles to induce humoral and cellular responses in mice
Chaiyaporn Kuwentrai, Jinming Yu, Rong Li, Bao‐Zhong Zhang, Ye‐Fan Hu, Hua‐Rui Gong, Ying Dou, Jian Deng, Jian‐Dong Huang, Chenjie Xu
Abstract
The S1 subunit of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein contains an immunogenic receptor-binding domain (RBD), which is a promising candidate for the development of a potential vaccine. This study demonstrated that intradermal delivery of an S-RBD vaccine using a dissolvable microneedle skin patch can induce both significant B-cell and significant T-cell responses against S-RBD. Importantly, the outcomes were comparable to that of conventional bolus injection.
Topics & Concepts
Spike ProteinIntradermal injectionReceptorProtein subunitCoronavirusImmunologySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)MedicineVirologyCoronavirus disease 2019 (COVID-19)BiologyChemistryPathologyGeneInternal medicineInfectious disease (medical specialty)BiochemistryDiseaseAdvancements in Transdermal Drug DeliveryDermatology and Skin DiseasesImmunotherapy and Immune Responses