Alternative metabolic pathways and strategies to high-titre terpenoid production in<i>Escherichia coli</i>
Mauro A. Rinaldi, Clara A. Ferraz, Nigel S. Scrutton
Abstract
expression. Mining the growing number of bacterial genomes for new bacterial terpenoid synthases and modifying enzymes identifies enzymes that outperform eukaryotic ones and expand microbial terpenoid production diversity. Terpenoid removal from cells is also crucial in production, and so terpenoid recovery and approaches to handle end-product toxicity increase titres. Combined, these strategies are contributing to current efforts to increase microbial terpenoid production towards commercial feasibility.
Topics & Concepts
TerpenoidMetabolic engineeringBiologyMevalonate pathwayMetabolic pathwayBiochemistryPrenylationEnzymeBiosynthesisPlant biochemistry and biosynthesisMicrobial Natural Products and BiosynthesisMicrobial Metabolic Engineering and Bioproduction