Transwell migration assay to interrogate human CAR-T cell chemotaxis
Arman Öner, Sebastian Kobold
Abstract
A major impediment to effective cellular therapies in solid tumors is the limited access of therapeutic cells to the tumor site. One strategy to overcome this challenge is to endow T cells with chemotactic properties required to access tumor tissue. Here, we present a chimeric antigen receptor (CAR)-modified T cell strategy centered around enhanced T cell trafficking. We outline isolation, activation, and transduction of human T cells, as well as techniques for assessing migratory and cytotoxic capacity of CAR-T cells. For complete details on the use and execution of this protocol, please refer to Lesch et al. (2021).
Topics & Concepts
ChemotaxisChimeric antigen receptorCytotoxic T cellCell biologyTransduction (biophysics)Cancer researchT cellBiologyChemistryReceptorImmunologyIn vitroImmune systemGeneticsBiochemistryCAR-T cell therapy researchNanowire Synthesis and ApplicationsAdvancements in Semiconductor Devices and Circuit Design