Development and validation of a high-throughput whole cell assay to investigate Staphylococcus aureus adhesion to host ligands
Laurenne E. Petrie, Allison C. Leonard, Julia L. Murphy, Georgina Cox
Abstract
Genetic Adhesion Network, which identified a core gene set involved in adhesion to all three host ligands, and unique genetic signatures. In summary, this assay will enable high-throughput chemical screens to identify anti-adhesives and our findings provide insight into the target space of such an approach.
Topics & Concepts
SortaseBacterial adhesinCell adhesionSortase AAdhesionBiologyStaphylococcus aureusMutantHigh-throughput screeningFibronectinCell adhesion moleculeCell biologyMicrobiologyComputational biologyGeneticsGeneCellChemistryExtracellular matrixEscherichia coliBacteriaOrganic chemistryBiochemical and Structural CharacterizationAntimicrobial Resistance in StaphylococcusToxin Mechanisms and Immunotoxins