A flexible kinetic assay efficiently sorts prospective biocatalysts for PET plastic subunit hydrolysis
Jessica Lusty Beech, Rita Clare, William M. Kincannon, Erika Erickson, J.E. McGeehan, Gregg T. Beckham, Jennifer L. DuBois
Abstract
stringency, finding the assay to be easily adaptable to diverse screening conditions and kinetic measurements. This method offers an accurate, easily accessible, and cost-effective route towards high-throughput library screening to support the discovery, directed evolution, and protein engineering of these critical biocatalysts.
Topics & Concepts
HydrolysisEsterasePolyethylene terephthalateChemistrySubstrate (aquarium)BioconversionEnzymeChromatographyEnzyme catalysisCombinatorial chemistryOrganic chemistryMaterials scienceGeologyOceanographyComposite materialFermentationbiodegradable polymer synthesis and propertiesMicroplastics and Plastic PollutionEnzyme Catalysis and Immobilization