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A cellular basis for heightened gut sensitivity in females

Archana Venkataraman, Eric E. Figueroa, Joel Castro, F Navarro, Deepanshu Soota, Stuart M. Brierley, David Julius, Holly A. Ingraham

2025Science11 citationsDOIOpen Access PDF

Abstract

Visceral pain disorders, such as irritable bowel syndrome, exhibit a marked female prevalence. Enhanced signaling between enterochromaffin (EC) cells in the gut epithelium and mucosal sensory nerve fibers likely contributes to this sex bias. We identified an estrogen-responsive paracrine pathway in which two enteroendocrine cell types, peptide YY (PYY)–expressing L cells and serotonergic EC cells, communicate to increase gut sensitivity in females. We demonstrate that estrogen signaling up-regulates the bacterial metabolite short-chain fatty acid receptor Olfr78 on colonic L cells, increasing PYY release and their sensitivity to acetate. Elevated PYY acts on neighboring EC cells by means of NPY1R, thereby enhancing serotonin release and gut pain. We propose that hormonal fluctuations, in conjunction with internal (stress) or environmental (diet) factors, amplify this local estrogen-responsive colonic circuit, resulting in maladaptive gut sensitivity.

Topics & Concepts

Enterochromaffin cellEnteroendocrine cellPeptide YYSerotonergicIrritable bowel syndromeSerotoninEndocrinologyInternal medicineBiologyParacrine signallingReceptorNeuroendocrine cellHormoneGut flora5-Hydroxyindoleacetic acidMetaboliteEstrogenEstrogen receptorCellGlycocalyxIleumCell biology5-HT receptorMonoamine neurotransmitterEpitheliumNeurotransmitterCell typeEndocrine systemChemistryIntestinal epitheliumVisceral painNecturusIntestinal mucosaBarostatGastrointestinal motility and disordersNeuropeptides and Animal PhysiologyHelicobacter pylori-related gastroenterology studies
A cellular basis for heightened gut sensitivity in females | Litcius