Cas12a/Guide RNA-Based Platforms for Rapidly and Accurately Identifying Staphylococcus aureus and Methicillin-Resistant S. aureus
Xiaoying Cao, Yanbin Chang, Chunqing Tao, Sen Chen, Qiuxia Lin, Chao Ling, Shifeng Huang, Hengshu Zhang
Abstract
The spread of methicillin-resistant Staphylococcus aureus (MRSA) poses a major threat to global health. Isothermal amplification combined with the trans -cleavage activity of Cas12a has been exploited to generate diagnostic platforms for pathogen detection.
Topics & Concepts
Recombinase Polymerase AmplificationCRISPRLoop-mediated isothermal amplificationStaphylococcus aureusTrans-activating crRNABiologyComputational biologyPolymerase chain reactionDNACas9GeneGeneticsBacteriaCRISPR and Genetic EngineeringBiosensors and Analytical DetectionAntimicrobial Resistance in Staphylococcus