Protocol for mouse adult neural stem cell isolation and culture
Ahmed K.M.A. Ahmed, Toke Jost Isaksen, Toshihide Yamashita
Abstract
This protocol entails a simple method for isolation, culturing, and in vitro differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells are an important in vitro model to investigate stem cell properties such as proliferation and differentiation and to expand the understanding of plasticity in the adult brain. For complete details on the use and execution of this protocol, please refer to Isaksen et al. (2020).
Topics & Concepts
Subventricular zoneNeural stem cellDentate gyrusStem cellBiologyNeurosphereNeurogenesisAdult stem cellNeuroscienceHippocampusIn vitroCell biologyEndothelial stem cellGeneticsNeurogenesis and neuroplasticity mechanismsPluripotent Stem Cells ResearchNerve injury and regeneration