Next-generation CRISPR/Cas-based ultrasensitive diagnostic tools: current progress and prospects
Deepak Kumar Sahel, Gangadari Giriprasad, Reena Jatyan, Sonia Guha, Aishwarya Korde, Anupama Mittal, Sunil Bhand, Deepak Chitkara
Abstract
-cleavage of a nonspecific fluorescent DNA/RNA probe, resulting in the production of a fluorescent signal that could be quantitatively recorded. Later, nanotechnology and mobile-based detection applications were incorporated into the system to develop advanced lateral flow-based strips and are also associated with the technology to make it more feasible. Overall, this review compiles the experimental evidence consolidating the application of CRISPR/Cas as next-generation biosensors for diagnostic applications.
Topics & Concepts
CRISPRGenome editingComputational biologyHomology directed repairHomology (biology)Computer scienceGeneNanotechnologyBiologyGeneticsMaterials scienceDNA repairDNA mismatch repairCRISPR and Genetic EngineeringInnovation and Socioeconomic DevelopmentRNA and protein synthesis mechanisms