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Electro-induced C-H/S-H cross-coupling for the functionalization/macrocyclization of cysteine-containing peptides

Fang Xiang, Jia Deng, Xianyong Bu, Youjun Zhou, Jianrong Zhang, Yue Weng, Meng Gao

2025Nature Communications8 citationsDOIOpen Access PDF

Abstract

Herein, an electro-induced umpolung approach that enables the efficient functionalization/macrocyclization of cysteine-containing peptides is reported. Notably, this method utilizes simple halogen source and takes metal-mediated halogen atom transfer as the main pathway to enable the in-situ polarity reversal, highlighting the unique possibilities associated with electrochemical activation methods. Under simple and mild conditions, cysteine residue can be well-labelled with high chemo-selectivity and excellent conversion. This transformation can tolerate a wide range of valuable enamines, azoles, and peptides partners, and can also be utilized as a macrocyclization tactic for cyclic peptide synthesis and other areas. In comparison to the well-developed electrochemical transformations of thiophenols, the electrochemical modification of peptides and proteins through cysteine remains rather underdeveloped. Here the authors report an electro-induced umpolung approach that enables the efficient functionalization/macrocyclization of cysteine-containing peptides.

Topics & Concepts

UmpolungChemistryCysteinePeptideResidue (chemistry)Combinatorial chemistryCyclic peptideHalogenPolarity (international relations)BiochemistryPeptide synthesisComputational biologyNanotechnologyAtom (system on chip)Transformation (genetics)Simple (philosophy)SulfurRadical Photochemical ReactionsSulfur-Based Synthesis TechniquesCatalytic C–H Functionalization Methods