Two-photon endomicroscopy with microsphere-spliced double-cladding antiresonant fiber for resolution enhancement
Conghao Wang, huilan liu, Haodong Cui, Jianrui Ma, Yijun Li, Jingquan Tian, Chaoyong Jin, Yanchuan Chen, Yuqian Gao, Qiang Fu, Yanhui Hu, Dakun Wu, Fei Yu, Runlong Wu, Aimin Wang, Lishuang Feng
Abstract
We demonstrate a miniature fiber-optic two two-photon endomicroscopy with microsphere-spliced double-cladding antiresonant fiber for resolution enhancement. An easy-to-operate process for fixing microsphere permanently in an antiresonant fiber core, by arc discharge, is proposed. The flexible fiber-optic probe is integrated with a parameter of 5.8 mm × 49.1 mm (outer diameter × rigid length); the field of view is 210 µm, the resolution is 1.3 µm, and the frame rate is 0.7 fps. The imaging ability is verified using ex-vivo mouse kidney, heart, stomach, tail tendon, and in-vivo brain neural imaging.