Fabrication of Tetra-Substituted Copper(II) Phthalocyanine-Graphene Modified Pencil Graphite Electrode for Amperometric Detection of Hydrogen Peroxide
Ozge Gorduk, Semih Gördük, Yücel Şahin
Abstract
In this study, tetra-substituted copper(II) phthalocyanine-graphene modified pencil graphite electrode (CuPc-Gr/PGE) was developed for the amperometric determination of hydrogen peroxide (H 2 O 2 ) for the first time in the literature. The developed non-enzymatic electrode platform was electrochemically characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods. Scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) methods were also used for surface characterization of the modified electrode. The response to H 2 O 2 at the working potential of −0.4 V is linear in the 0.1 μ M to 100 μ M concentration range in PBS pH 7.0. The limit of detection (LOD) and limit of quantitation (LOQ) are 0.045 and 0.15 μ M, respectively. Good selectivity was observed within physiological interference species such as ascorbic acid, dopamine, uric acid, and glucose. Additionally, amount of H 2 O 2 was determined using the novel produced non-enzymatic sensor with satisfactory results in beverage samples. These consequences imply that the CuPc-Gr/PGE displays effective electrocatalytic activity for the reduction of H 2 O 2 , indicating it has capability use for the detection of H 2 O 2 .