Visualization of carboxylesterase 2 with a near-infrared two-photon fluorescent probe and potential evaluation of its anticancer drug effects in an orthotopic colon carcinoma mice model
Yan Wang, Feifei Yu, Feifei Yu, Xianzhu Luo, Mingshun Li, Linlu Zhao, Fabiao Yu, Fabiao Yu
Abstract
We establish a near-infrared two-photon fluorescent probe for the detection of CE2 with high selectivity and sensitivity. This probe exhibits low cytotoxicity and superior tissue penetration ability for evaluating the real-time activity of CE2 in living cells, in cancer tissues, and in a colon carcinoma mice model.
Topics & Concepts
FluorescenceChemistryCarboxylesteraseTwo-photon excitation microscopyInfraredColon carcinomaSelectivityDrugBiophysicsColorectal cancerPharmacologyBiochemistryCancerBiologyEnzymePhysicsOpticsCatalysisGeneticsMolecular Sensors and Ion DetectionCholinesterase and Neurodegenerative DiseasesElectrochemical sensors and biosensors