The Role of miRNA‐146a and Proinflammatory Cytokines in Carotid Atherosclerosis
Pan Huang, Xiaoying He, Min Xu
Abstract
The aim of this study was to investigate the expression and significance of miRNA‐146a in peripheral blood of patients with carotid atherosclerosis (CAS). Patients with CAS were selected as the stenosis (CAS) group ( n = 180). According to the degree of stenosis, they were divided into the mild stenosis group (MS group, n = 64), moderate stenosis group (M group, n = 62 cases), and severe stenosis group (SS group, n = 54). According to the plaque type, patients were divided into a stable plaque group (SP group, n = 96) and a vulnerable plaque group (VP group, n = 84). Patients without CAS represented the normal group ( n = 90). The expression levels of miRNA‐146a as well as IL‐6 and TNF‐ α in peripheral blood were detected by RT‐PCR and ELISA, respectively. The expression levels of miRNA‐146a, IL‐6, and TNF‐ α in the CAS group were higher than those in the normal group and positively correlated with the degree of stenosis and plaque vulnerability. The expression levels of miRNA‐146a, IL‐6, and TNF‐ α in the stable plaque group were lower than those in the vulnerable plaque group. The area under the curve of miRNA‐146a predicting the vulnerability of plaques was significant at 0.641. The expression level of miRNA‐146a in the CAS group was positively correlated with IL‐6 and TNF‐ α levels. Our results indicate that miRNA‐146a may participate in the occurrence and development of CAS by regulating the expression of IL‐6 and TNF‐ α , which may be potential biomarkers of CAS.