Combining portable NIR spectroscopy and multivariate calibration for the determination of ethanol in fermented alcoholic beverages by a multi-product model
Ana Carolina da Costa Fulgêncio, Glaucimar Alex Passos Resende, Marden Claret Fontoura Teixeira, Bruno Gonçalves Botelho, Marcelo M. Sena
Abstract
In this study, a multivariate calibration multi-product model was built by combining partial least square regression (PLS) and portable near infrared (NIR) spectroscopy for the determination of ethanol content in fermented alcoholic beverages. Reference values were obtained by gas chromatography with flame ionization detection (GC-FID). Aiming at building a robust model, a great variety of beers, ciders, meads, and wines were incorporated into the model. NIR spectra were recorded between 908 and 1676 nm for 153 alcoholic beverage samples, corresponding to a range from 4.3 to 15.3% (v/v) of alcohol content. PLS model provided accurate results with root mean square errors of calibration (RMSEC) and prediction (RMSEP) of 0.8% and 0.9%, respectively. The developed method was validated through the estimate of proper figures of merit, such as linearity, trueness, precision, analytical sensitivity, bias, and residual prediction deviation (RPD). This method was simple, direct, rapid, of low-cost and environmentally friendly, not consuming reagents or solvents nor generating chemical waste. It could be incorporated in analytical platforms for quality inspection, contributing to provide better transparency in the food supply chain.