Formate Dehydrogenase: Recent Developments for NADH and NADPH Recycling in Biocatalysis
Artur Maier, Lindelo M. Mguni, Anna Ngo, Dirk Tischler
Abstract
Abstract Formate dehydrogenases (FDHs) catalyze the oxidation of formate to CO 2 while reducing NAD(P) + to NAD(P)H and are classified into two main classes: metal‐dependent (Mo‐ or W‐containing) and metal‐independent FDHs. The latter are oxygen‐tolerant and relevant as a cofactor regeneration system for various bioprocesses and gained more and more attention due to their ability to catalyze the reverse CO 2 reduction. This review gives an overview of metal‐independent FDHs, the recent advances made in this field, and their relevance for future applications in biocatalysis. This includes the exploitation of novel FDHs which have altered co‐substrate specificity as well as enzyme engineering approaches to improve process stability and general performance.