Evaluation of ID Fungi Plates Medium for Identification of Molds by MALDI Biotyper
M Robert, Charlotte Romero, Céline Dard, Cécile Garnaud, Odile Cognet, Thomas Girard, Tahinamandranto Rasamoelina, Muriel Cornet, Danièle Maubon
Abstract
MALDI-TOF mass spectrometry (MS) identification of pathogenic filamentous fungi is often impaired by difficulties in harvesting hyphae embedded in the medium and long extraction protocols. The ID Fungi Plate (IDFP) is a novel culture method developed to address such difficulties and improve the identification of filamentous fungi by MALDI-TOF MS. We cultured 64 strains and 11 clinical samples on IDFP, Sabouraud agar-chloramphenicol (SAB), and ChromID Candida agar (CAN2). We then compared the three media for growth, ease of harvest, amount of material picked, and MALDI-TOF identification scores after either rapid direct transfer (DT) or a long ethanol-acetonitrile (EA) extraction protocol.