RNA-Seq Data Analysis in Galaxy
Bérénice Batut, Marius van den Beek, Maria Doyle, Nicola Soranzo
Abstract
A complete RNA-Seq analysis involves the use of several different tools, with substantial software and computational requirements. The Galaxy platform simplifies the execution of such bioinformatics analyses by embedding the needed tools in its web interface, while also providing reproducibility. Here, we describe how to perform a reference-based RNA-Seq analysis using Galaxy, from data upload to visualization and functional enrichment analysis of differentially expressed genes.
Topics & Concepts
Computer scienceUploadRNA-SeqComputational biologySoftwareVisualizationEmbeddingRNAInterface (matter)Data miningInformation retrievalBiologyGeneWorld Wide WebTranscriptomeGeneticsGene expressionArtificial intelligenceProgramming languageOperating systemBubbleMaximum bubble pressure methodGenomics and Phylogenetic StudiesMolecular Biology Techniques and ApplicationsRNA modifications and cancer