The Development of an Amperometric Enzyme Biosensor Based on a Polyaniline‐Multiwalled Carbon Nanocomposite for the Detection of a Chemotherapeutic Agent in Serum Samples from Patients
Ahmad Gholami, Fatemeh Farjami, Younes Ghasemi
Abstract
Polyaniline was electrochemically polymerized onto the platinum electrode modified with a mixture of Nafion and multiwalled carbon nanotube (PANI/MWCNT/Nf/Pt) to detect ammonium ion. The nanobiosensor (ASNase/PANI/MWCNT/Nf/Pt) was then prepared by immobilizing L‐asparaginase (L‐ASNase) on the PANI/MWCNT/Nf nanocomposite. The prepared nanobiosensor was used for the rapid and sensitive detection of serum concentration of the anticancer agent L‐asparagine (L‐Asp) during chemotherapy. The nanobiosensor has dynamic ranges of zero to 180 μ M. The sensitivity of the nanobiosensor was 0.829 μ A μ M −1 cm −2 , and the response time was less than 30 s. The detection limit was 140 nM of L‐Asp. The Michaelis–Menten constant ( K m ) was measured to be 36.2 mM. The nanobiosensor was successfully applied for the determination of L‐Asp in the blood samples of leukemia patients.