Engineering a Highly Efficient Carboligase for Synthetic One-Carbon Metabolism
Maren Nattermann, Simon Burgener, Pascal Pfister, Alexander Chou, Luca Schulz, Seung Hwan Lee, Nicole Paczia, Jan Zarzycki, Ramón González, Tobias J. Erb
Abstract
. In addition to the increased GCS activity, MeOXC4 showed up to 300-fold higher activity than the wild type toward a broad range of carbonyl acceptor substrates. When applied in vivo, MeOXC4 enables the production of glycolate from formaldehyde, overcoming the current bottleneck of C1-C1 condensation in whole-cell bioconversions and paving the way toward synthetic C1 assimilation routes in vivo.
Topics & Concepts
ChemistrySaturated mutagenesisMetabolic engineeringProtein engineeringEnzymeSynthetic biologyEscherichia coliCombinatorial chemistryBiochemistryBiologyComputational biologyGeneMutantBiochemical Acid Research StudiesNeurological diseases and metabolismBiochemical and Molecular Research