Feasibility and barriers to rapid establishment of patient-derived primary osteosarcoma cell lines in clinical management
Thomas Chow, William Humble, Enrico Lucarelli, Carmine Onofrillo, Peter Choong, Claudia Di Bella, Serena Duchi
Abstract
results. Developing a single-site laboratory protocol to rapidly establish patient-derived primary cancer cell lines (PCCL) within a clinically actionable time frame of a few weeks will have significant scientific and clinical ramifications. These PCCL can widen the pool of available cell lines for study while patient-specific data could derive therapeutic correlation. This endeavor is exceedingly challenging considering the proposed time constraints. By proposing key definitions and a clear theoretical framework, this evaluation of osteosarcoma cell line establishment methodology over the past three decades assesses feasibility by identifying barriers and suggesting solutions, thereby facilitating systematic experimentation and optimization.