Detection of Proteus Mirabilis Carrying blaCTX-M, blaSHV, and blaTEM Genes Related to Urinary Tract Infections
Miaad K. Alkhudhairy, Mahasin Sifir Madkhur
Abstract
Background: Proteus mirabilis is a bacterium that causes many serious systematic infections. The aim of the study: Determination of Proteus mirabilis carrying blaCTX-M, blaSHV, and blaTEM genes related to urinary tract infections. Materials and Methods: 402 samples of urine were randomly collected from patients with symptomatic urinary tract infections at Al-Sadder Hospital in Al-Najaf Governorate, Iraq, from October 2022 to December 2023. Results: 71 (17.7%) isolates of P. mirabilis were identified according to microscopic, culture, and biochemical characteristics, then were tested for their ability to produce extended-spectrum β-lactamases by the two phenotypic methods: double disc synergy test and modified Hodge test. 19 (26.8%) extended-spectrum β-lactamases-producers, which had previously been evaluated using the phenotypic method, were subjected to the investigation of β-lactamases encoding genes by using the polymerase chain reaction technique. This molecular technique detected 8 (42.1%) isolates carrying single β-lactamases encoding gene, 7 (36.8%) isolates had blaCTX-M gene, only one isolate (5.3%) carried blaSHV gene, and no single blaTEM gene was detected in any isolate. 3 (15.8%) isolates harbored multiple genes: blaCTX-M and blaTEM. Conclusions: blaCTX-M gene was found to be more predominant among the extended-spectrum β-lactamases-producer under study than other genes