M-MuLV reverse transcriptase: Selected properties and improved mutants
Igor P. Oscorbin, М. Л. Филипенко
Abstract
Reverse transcriptases (RTs) are enzymes synthesizing DNA using RNA as the template and serving as the standard tools in modern biotechnology and molecular diagnostics. To date, the most commonly used reverse transcriptase is the enzyme from Moloney murine leukemia virus, M-MuLV RT. Since its discovery, M-MuLV RT has become indispensable for modern RNA studies; the range of M-MuLV RT applications is vast, from scientific tasks to clinical testing of human pathogens. This review will give a brief description of the structure, thermal stability, processivity, and fidelity, focusing on improving M-MuLV RT for practical usage.
Topics & Concepts
Reverse transcriptaseProcessivityMurine leukemia virusRNAComputational biologyVirologyHuman immunodeficiency virus (HIV)BiologyMutantDNADNA polymeraseVirusGeneticsGeneHIV Research and TreatmentBacteriophages and microbial interactionsRNA and protein synthesis mechanisms