Reproducibility and sensitivity of 36 methods to quantify the SARS-CoV-2 genetic signal in raw wastewater: findings from an interlaboratory methods evaluation in the U.S.
Brian M. Pecson, Emily Darby, Charles N. Haas, Yamrot M. Amha, Mitchel Bartolo, Richard E. Danielson, Yeggie Dearborn, George di Giovanni, Christobel Ferguson, Stephanie Fevig, Erica Gaddis, Donald Gray, George Lukasik, Bonnie Mull, Liana Olivas, Adam W. Olivieri, Yan Qu, SARS-CoV-2 Interlaboratory Consortium
Abstract
range of recovery efficiency and limit of detection highlighting the importance of recovery correction and the need to consider method sensitivity when selecting methods for wastewater surveillance.
Topics & Concepts
ReproducibilityWastewaterSensitivity (control systems)Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Coronavirus disease 2019 (COVID-19)2019-20 coronavirus outbreakEnvironmental scienceMedicineChromatographyEngineeringVirologyEnvironmental engineeringChemistryInternal medicineElectronic engineeringInfectious disease (medical specialty)OutbreakDiseaseSARS-CoV-2 detection and testingViral gastroenteritis research and epidemiologyBiosensors and Analytical Detection