Discovery proteomic (DIA) LC-MS/MS data acquisition and analysis v2
Yan Chen, Jennifer Gin, Christopher J. Petzold
Abstract
This protocol details steps in discovery proteomic data-independent acquisition with a standard-flow UHPLC-Obitrap system and a subsequent DIA-NN library-free database search. The data acquisition method was adapted from González Fernández-Niño, S. M., et al. "Standard flow liquid chromatography for shotgun proteomics in bioenergy research."Frontiers in bioengineering and biotechnology, 3 (2015): 44.
Topics & Concepts
Shotgun proteomicsProteomicsData acquisitionShotgunComputer scienceChromatographyComputational biologyChemistryDatabaseBiologyOperating systemGeneBiochemistryAdvanced Proteomics Techniques and ApplicationsMass Spectrometry Techniques and ApplicationsMetabolomics and Mass Spectrometry Studies