Rapid no-wash labeling of PYP-tag proteins with reactive fluorogenic ligands affords stable fluorescent protein conjugates for long-term cell imaging studies
Naresh Kumar, Yuichiro Hori, Miyako Nishiura, Kazuya Kikuchi
Abstract
studies demonstrating that PYP-DMAC-CMK conjugates exhibit excellent photostability and cellular stability profiles which enables them to be used for long-term protein imaging studies in cellular systems. The potential of using this no wash fluorescent labeling PYP-tag-DMAC system to visualise dividing cells undergoing mitosis and for imaging a PYP-tag fused telomere binding protein bound to chromatin in cell nuclei has been demonstrated.
Topics & Concepts
ThioetherChemistryFluorescenceCovalent bondConjugateCombinatorial chemistryTarget proteinProtein tagFluorescent proteinBiochemistryGreen fluorescent proteinStereochemistryRecombinant DNAOrganic chemistryGeneFusion proteinPhysicsMathematicsMathematical analysisQuantum mechanicsClick Chemistry and ApplicationsBiotin and Related StudiesUbiquitin and proteasome pathways