In-solution buffer-free digestion allows full-sequence coverage and complete characterization of post-translational modifications of the receptor-binding domain of SARS-CoV-2 in a single ESI–MS spectrum

Luis Ariel Espinosa, Yassel Ramos, Iván Andújar, Enso Onill Torres, Gleysin Cabrera, A.M. Martin, Diamilé Roche, Glay Chinea, Mónica Becquet, Isabel González, Camila Canaán-Haden, Elías Nelson, Gertrudis Rojas, Beatriz Pérez-Massón, Dayana Pérez‐Martínez, Tammy Boggiano-Ayo, Julio Palacio, Sum Lai Lozada Chang, Lourdes Hernández, Kathya Rashida de la Luz Hernández, Saloheimo Markku, Marika Vitikainen, Yury Valdés-Balbín, Darielys Santana-Medero, Daniel G. Rivera, Vicente Vérez-Bencomo, Mark Emalfarb, Ronen Tchelet, Gerardo Guillén, Miladys Limonta‐Fernández, Eulogio Pimentel, Marta Ayala, Vladimir Besada, Luis Javier González